| Player | # | Pos | HT | WT | Age | Exp | College |
|---|---|---|---|---|---|---|---|
| Lorenzo Alexander | 57 | LB | 6-1 | 245 | 36 | 13 | California |
| Maurice Alexander | 41 | LB | 6-2 | 220 | 28 | 5 | Utah State |
| Josh Allen | 17 | QB | 6-5 | 237 | 23 | 2 | Wyoming |
| Matt Barkley | 5 | QB | 6-2 | 234 | 28 | 7 | Southern California |
| Cole Beasley | 10 | WR | 5-8 | 174 | 30 | 8 | Southern Methodist |
| Nate Becker | 84 | TE | 6-5 | 264 | 23 | R | Miami (Ohio) |
| Russell Bodine | 66 | C | 6-3 | 308 | 27 | 6 | North Carolina |
| Ike Boettger | 65 | G | 6-6 | 313 | 24 | 2 | Iowa |
| Corey Bojorquez | 9 | P | 6-0 | 208 | 22 | 2 | New Mexico |
| Victor Bolden Jr. | 13 | WR | 5-8 | 178 | 24 | 3 | Oregon State |
| John Brown | 2 | WR | 5-11 | 178 | 29 | 6 | Pittsburg State |
| Rafael Bush | 20 | S | 5-11 | 203 | 32 | 9 | South Carolina State |
| Cory Carter | 7 | P | 5-11 | 220 | 25 | 2 | Texas Southern |
| Jason Croom | 80 | TE | 6-5 | 246 | 25 | 2 | Tennessee |
| Dion Dawkins | 73 | T | 6-5 | 320 | 25 | 3 | Temple |
| Patrick DiMarco | 42 | FB | 6-1 | 234 | 30 | 8 | South Carolina |
| Tyrel Dodson | 53 | LB | 6-0 | 237 | 21 | R | Texas A&M |
| Vladimir Ducasse | 62 | G | 6-5 | 329 | 31 | 10 | Massachusetts |
| Nick Easley | 86 | WR | 5-11 | 203 | 22 | R | Iowa |
| Tremaine Edmunds | 49 | LB | 6-5 | 250 | 21 | 2 | Virginia Tech |
| Jon Feliciano | 76 | G | 6-4 | 325 | 27 | 5 | Miami (Fla.) |
| Reid Ferguson | 69 | LS | 6-2 | 235 | 25 | 3 | Louisiana State |
| Juwan Foggie | 48 | LB | 6-0 | 235 | 23 | R | UNC Charlotte |
| Cody Ford | 70 | T | 6-3 | 329 | 22 | R | Oklahoma |
| Robert Foster | 16 | WR | 6-2 | 196 | 25 | 2 | Alabama |
| E.J. Gaines | 26 | CB | 5-10 | 190 | 27 | 6 | Missouri |
| Frank Gore | 28 | RB | 5-9 | 212 | 36 | 15 | Miami (Fla.) |
| Eli Harold | 59 | DE | 6-3 | 255 | 25 | 5 | Virginia |
| Stephen Hauschka | 4 | K | 6-4 | 210 | 34 | 12 | Middlebury |
| Jerry Hughes | 55 | DE | 6-2 | 254 | 30 | 10 | Texas Christian |
| Micah Hyde | 23 | S | 6-0 | 197 | 28 | 7 | Iowa |
| Tyree Jackson | 6 | QB | 6-7 | 249 | 21 | R | Buffalo |
| Darryl Johnson | 92 | DE | 6-6 | 253 | 22 | R | North Carolina A&T |
| Jaquan Johnson | 46 | S | 5-10 | 191 | 23 | R | Miami (Fla.) |
| Kevin Johnson | 36 | CB | 6-0 | 185 | 26 | 5 | Wake Forest |
| Taron Johnson | 24 | CB | 5-11 | 192 | 22 | 2 | Weber State |
| Zay Jones | 11 | WR | 6-2 | 200 | 24 | 3 | East Carolina |
| Vosean Joseph | 50 | LB | 6-1 | 230 | 21 | R | Florida |
| Dawson Knox | 88 | TE | 6-4 | 254 | 22 | R | Mississippi |
| Tyler Kroft | 81 | TE | 6-6 | 252 | 26 | 5 | Rutgers |
| Deon Lacey | 44 | LB | 6-3 | 235 | 28 | 3 | West Alabama |
| Shaq Lawson | 90 | DE | 6-3 | 267 | 25 | 4 | Clemson |
| Cam Lewis | 47 | CB | 5-9 | 183 | 22 | R | Buffalo |
| Ryan Lewis | 38 | CB | 6-0 | 185 | 25 | 2 | Pittsburgh |
| Spencer Long | 61 | C/G | 6-5 | 318 | 28 | 6 | Nebraska |
| Star Lotulelei | 98 | DT | 6-2 | 315 | 29 | 7 | Utah |
| Mike Love | 56 | DE | 6-3 | 255 | 25 | 1 | South Florida |
| Dean Marlowe | 31 | S | 6-1 | 208 | 26 | 3 | James Madison |
| Ray-Ray McCloud III | 14 | WR | 5-9 | 190 | 22 | 2 | Clemson |
| LeSean McCoy | 25 | RB | 5-11 | 210 | 30 | 11 | Pittsburgh |
| Conor McDermott | 68 | T | 6-8 | 305 | 26 | 3 | UCLA |
| Garrett McGhin | 64 | OL | 6-6 | 316 | 23 | R | East Carolina |
| Isaiah McKenzie | 19 | WR | 5-8 | 173 | 24 | 3 | Georgia |
| Chase McLaughlin | 18 | K | 5-11 | 187 | 23 | R | Illinois |
| Matt Milano | 58 | LB | 6-0 | 223 | 24 | 3 | Boston College |
| Mitch Morse | 60 | C | 6-6 | 305 | 27 | 5 | Missouri |
| Marcus Murphy | 22 | RB | 5-9 | 195 | 28 | 4 | Missouri |
| Trent Murphy | 93 | DE | 6-6 | 260 | 28 | 6 | Stanford |
| Siran Neal | 33 | S | 6-0 | 206 | 24 | 2 | Jacksonville State |
| Ty Nsekhe | 77 | T | 6-8 | 330 | 33 | 5 | Texas State |
| Ed Oliver | 91 | DT | 6-1 | 287 | 21 | R | Houston |
| Kyle Peko | 94 | DT | 6-1 | 305 | 25 | 2 | Oregon State |
| Senorise Perry | 32 | RB | 6-0 | 210 | 27 | 5 | Louisville |
| Cam Phillips | 87 | WR | 6-0 | 201 | 23 | 1 | Virginia Tech |
| Harrison Phillips | 99 | DT | 6-3 | 307 | 23 | 2 | Stanford |
| Jordan Phillips | 97 | DT | 6-6 | 341 | 26 | 5 | Oklahoma |
| Lafayette Pitts | 30 | CB | 5-11 | 195 | 26 | 4 | Pittsburgh |
| Jordan Poyer | 21 | S | 6-0 | 191 | 28 | 7 | Oregon State |
| Denzel Rice | 37 | CB | 6-0 | 185 | 26 | 3 | Coastal Carolina |
| Andre Roberts | 8 | WR | 5-11 | 195 | 31 | 10 | The Citadel |
| Da’Mari Scott | 15 | WR | 6-0 | 205 | 23 | 2 | Fresno State |
| David Sills | 1 | WR | 6-3 | 211 | 23 | R | West Virginia |
| Devin Singletary | 40 | RB | 5-7 | 203 | 21 | R | Florida Atlantic |
| Jeremiah Sirles | 71 | T/G | 6-6 | 315 | 27 | 6 | Nebraska |
| Lee Smith | 85 | TE | 6-6 | 265 | 31 | 9 | Marshall |
| Quinton Spain | 67 | G | 6-4 | 330 | 27 | 5 | West Virginia |
| Julian Stanford | 51 | LB | 6-1 | 230 | 28 | 6 | Wagner |
| Tommy Sweeney | 89 | TE | 6-5 | 251 | 24 | R | Boston College |
| Wyatt Teller | 75 | G | 6-4 | 314 | 24 | 2 | Virginia Tech |
| Robert Thomas | 96 | DT | 6-1 | 316 | 28 | 3 | Arkansas |
| Corey Thompson | 52 | LB | 6-1 | 222 | 25 | 2 | Louisiana State |
| Keith Towbridge | TE | 6-5 | 262 | 24 | 2 | Louisville | |
| LaAdrian Waddle | 74 | T | 6-6 | 315 | 27 | 7 | Texas Tech |
| Christian Wade | 45 | RB | 5-9 | 196 | 28 | 1 | St. Mary’s University |
| Levi Wallace | 39 | CB | 6-0 | 179 | 24 | 2 | Alabama |
| L.T. Walton | 63 | DT | 6-5 | 305 | 27 | 5 | Central Michigan |
| De’Ondre Wesley | 79 | T | 6-6 | 331 | 26 | 3 | Brigham Young |
| Tre’Davious White | 27 | CB | 5-11 | 192 | 23 | 3 | Louisiana State |
| Duke Williams | 82 | WR | 6-3 | 225 | 26 | 1 | Auburn |
| Eddie Yarbrough | 54 | DE | 6-3 | 259 | 26 | 3 | Wyoming |
| T.J. Yeldon | 29 | RB | 6-1 | 223 | 25 | 5 | Alabama |
Bills Roster
